| 摘要: |
| 目的探讨电刺激大鼠小脑顶核(cerebellar fastigial nucleus,CFN)对视网膜缺血-再灌注损伤的保护作用及其机制.方法大鼠随机均分为三组,缺血-再灌注损伤组、缺血-再灌注治疗组和假手术组.用TUNEL试剂盒检测视网膜神经节细胞凋亡率;用免疫组织化学染色法检测视网膜细胞Bcl-2蛋白和Bax蛋白的表达.结果缺血-再灌注损伤可使视网膜神经节细胞发生凋亡(P<0.05),同时视网膜细胞Bcl-2蛋白表达逐渐减弱(P<0.05),Bax蛋白表达逐渐增强(P<0.05);而使用电刺激小脑顶核的治疗组上述改变较轻(P<0.05).结论电刺激大鼠小脑顶核对视网膜缺血-再灌注损伤具有保护作用,其机制可能通过上调Bcl-2蛋白表达,下调Bax蛋白表达来减少视网膜细胞的凋亡. |
| 关键词: 缺血-再灌注 电刺激 小脑顶核 凋亡 TUNEL Bcl-2 Bax 视网膜 |
| DOI： |
| 分类号:R774 |
| 基金项目: |
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| Electrical Stimulating Cerebellar Fastigial Nucleus Protects Retina against Ischemia/reperfusion Injury in Rats |
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LUO Zhe-wen,ZHANG Hong
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LUO Zhe-wen1),ZHANG Hong2)(1)Dept.of Ophthalmology,The Second People's Hospital of Yunnan Province,Kunmin Yunnan 650021,2)Dept.of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Hubei 430030,China
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| Abstract: |
| Objective To investigate the protective effect and mechanism of electrical stimulating cerebellar fastigial nucleus on retina against ischemia/reperfusion injury in rats.Methods Rats were divided randomly into three groups:group1(ischemia-reperfusion),group2(electrical-stimulating-treated),and group3(sham-operated).Apoptosis of retinal ganglion cells was detected by TUNEL method,and expression of Bcl-2 and Bax were measured by immunohistochemical staining methods.Results Ischemia/reperfusion injury induced ... |
| Key words: Ischemia-reperfusion injury Electrical stimulation Cerebellar fastigial nucleus Apoptosis TUNEL Bcl-2 Bax Retina |