| 摘要: |
| ［摘要］目的 构建肾上腺醛固酮腺瘤大鼠模型．方法 16只SD大鼠随机分对照组和醛固酮腺瘤组，在ALZET 2004微量渗透泵储药仓内分别注入醛固酮溶液或空白溶剂，然后将其埋于大鼠背部皮下．ALZET 2004微量渗透泵可以持续灌注4周，为使醛固酮作用时间达到8周，4周后更换渗透泵1次，同时尾套法测量大鼠尾动脉收缩压；8周后，放免法检测血浆醛固酮浓度和肾素活性．结果 3周后，与对照组大鼠相比，腺瘤组大鼠收缩压开始升高，第7周达到顶峰，之后维持在高水平；腺瘤组大鼠血浆醛固酮的浓度显著升高（P < 0.01），肾素活性被抑制（P < 0.01）．结论 大鼠皮下埋植微量渗透泵灌注醛固酮，可以成功构建大鼠醛固酮腺瘤模型. |
| 关键词: ［关键词］醛固酮腺瘤 微量渗透泵 动物模型 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Establishment of a Rat Model of Aldosterone-Producing Adenoma |
|
YAN Yong－ji
|
| Abstract: |
| ［Abstract］Objective To establish a rat model of aldosterone-producing adenoma（APA）. Methods 16 Sprague-Dawley rats subcutaneously implanted with an osmotic mini-pump，were randomly divided into 2 groups： control group（vehicle）and APA（1 μg/h）group．Because the mini-pump（ALZET 2004）could continuously infuse test substance for 4 weeks，another minipump was replaced after the fourth week to make the duration of aldosterone infusion for 8 weeks．Systolic blood pressure（SBP）was measured weekly by the tail-cuff method．At the termination of the study，blood was collected for measurements of plasma renin activity（PRA）and aldosterone concontration（PAC）with radioimmunoassay kits．Results After 3 weeks，APA rats showed significantly and progressively increased SBP compared with controls（P＜0.05），and the SBP level reached peak on 7th week and remained high levels．PRA was substantially depressed and PAC was significantly raised in APA rats（P＜0.01）． Conclusion By implanting osmotic minipumps subcutaneously， the rat models of APA can be successfully established， which provide a good platform for investigating the pathogenesis of APA. |
| Key words: ［Key words］Aldosterone-producing adenoma Osmotic pump Animal model |