| 摘要: |
| ［摘要］目的 探讨缺血后处理对猪非梗死区心肌细胞凋亡的影响．方法 滇南小耳猪15只，随机分为3组．（1） 假手术组（S组，n＝5），未行冠脉封堵及心肌缺血；（2）缺血再灌注组（I/R组，n＝5），心肌缺血60 min后，不行缺血后处理，直接撤除球囊；（3） 缺血后处理组（Postcond组，n＝5），心肌缺血60 min后，通过球囊充气和放气造成8次30 s/30 s短暂缺血再灌注．再灌注72 h采用2，3，5氯化三苯基氯四氮唑（TTC）染色确定心肌梗死面积；在猪非心肌梗死区，采用TUNEL法检测心肌凋亡细胞及免疫组化检测Bcl-2和Bax的蛋白表达．结果 再灌注72 h，缺血后处理组心肌梗塞面积、非心肌梗死区细胞凋亡指数及Bax的蛋白表达和Bax/Bcl-2的比值明显低于再灌注组（P＜0.05）．结论 缺血后处理可能通过减轻非梗死区心肌细胞凋亡发挥心肌保护作用． |
| 关键词: ［关键词］缺血后处理 心肌梗死 细胞凋亡 |
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| Effects of Ischemic Postconditioning on Myocardial Apoptosis in Non-infarct Zone in Pigs with Myocardium |
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SUN Hai－mei
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| Abstract: |
| ［Abstract］Objective To investigate the effect of ischemic postconditioning on myocardial apoptosis in non-infarct zone in pigs with myocardium．Methods Fifteen Diannan small-ear pigs were divided into three groups（n＝5 pre group） randomly：（1）Sham-operated（S）group：A balloon catheter was positioned in the mid-distal left anterior descending（LAD）via right femoral artery under fluoroscopic guidance，without ischemia；（2）Ischemia-reperfusion （I/R） group: the balloon was inflated and occluded the LAD for 60 minutes；（3）ischemic postconditioning（Postcond）group: pigs were treated the same as I/R group and 8 cycles of 30 s R and 30 s I before reperfusion. After 72 h of reperfusion，infarction size were measured by 2，3，5－triphenyltetrazolium chloride（TTC）staining．The apoptotic cells in the non-infarct zone myocardium was determined with terminal deoxynucleotidyl transferase-mediated dUTP-fluorescence in nick end（TUNEL） method，and apoptosis-related molecules were studied by immunohistochemical analysis. Result After 72h of reperfusion，the infarction size，apoptotic index（AI）of non-infarcted zone，the levels of Bax protein expression，and Bax/Bcl-2 ratio were significantly lower in Postcond group than those in I/R group（P＜0.05）．Conclusion Ischemic postconditioning can reduce myocardial apoptosis in non-infarct zone and maybe play a myocardial protection effect. |
| Key words: ［Key words］Ischemic postconditioning Myocardial infarction Apoptosis |