| 摘要: |
| ［摘要］ 目的 探讨昆明地区汉族人群维生素D受体基因多态性与2型糖尿病的相关性．方法 选取昆明地区汉族2型糖尿病患者（DM组）90例及健康对照（NC组）36例．运用PCR-FRLP技术测定维生素D受体ApaI基因多态性，比较各组基因型及等位基因频率及不同基因型间相关临床资料．结果 （1）2型糖尿病组与正常组间维生素D受体Apal基因存在多态性，2型糖尿病组中C/A基因型分布频率明显高于正常对照组（χ2＝53.432，P＜0.001）；（2）2型糖尿病组的年龄、FPG、LDL-C高于正常对照组，25-OHD3、Ca低于正常对照组（P＜0.05）；糖尿病组A/A基因型Scr显著高于其他组别，差异有统计学意义（P＜0.05）；3组基因型间UA均存在统计学差异（P＜0.05），且C/A基因型最高；（3）二分类Logistic回归分析结果提示ApaI C/A基因型（OR＝1.36， 95%CI： 0.54-1.71）、年龄（OR＝1.09，95% CI： 1.03-1.18 ）为2型糖尿病的危险因素，而25-OHD3 （OR＝0.84，95%CI：0.73～0.93）可能是T2DM的发生的保护因素．结论 （1）昆明地中VDR ApaI位点 C/A基因型与T2DM的发生存在相关性；（3）25-OHD3是T2DM的发生的保护因素，增龄是2型糖尿病的危险因素． |
| 关键词: ［关键词］ 2型糖尿病 维生素D受体基因 聚合酶链反应限制性片段长度多态性 昆明 |
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| 基金项目:［基金项目］ 云南省教育厅研究生基金资助项目（2013J054） |
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| The Correlation between Vitamin D Receptor Gene Polymorphism and Type 2 Diabetes Mellitus of the Han Nationality in Kunming Region |
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DU Si－Cheng1,2,3
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1.（1）Dept. of Gerontology，The First Affiliated Hospital of Kunming Medical University;2）Diabetes Department;3）Clinical Laboratory Department，Kunming Yunnan 650032，China）
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| Abstract: |
| ［Abstract］Objective To explore the correlation between vitamin D receptor （VDR） gene polymorphism and type 2 diabetes mellitus of the Han nationality in Kunming region. Methods One-hundred-twenty-six Kunming individuals（90 T2DMs and 36 controls）were enrolled in this randomized controlled trial. rs7975232 single nucleotide polymorphism （SN+J8Ps） of the VDR was tested by using Polymerase chain reaction of restriction fragment length polymorphism（PCR-RFLP）， the genotype and its frequencies were compared between the two groups．Results（1）There were statistically significant association in three kinds of genotype of ApaI of VDR gene polymorphism between two groups（P＜0.001）. The C/A genotype was more common in T2DM patients （62.2% vs 38.9%）. （2）Statistic analysis software showed that the frequency of C/A genotype of ApaI was higher than that in the control group（OR=1.36， 95%CI: 0.54-1.71）. Whats more， age in T2DM group were also higher than that in the control group（OR= 1.09，95% CI: 1.03-1.18 ）. 25OHD3 in T2DM group was lower than that in the control group （OR=0.84，95%CI:0.73-0.93），suggesting that C/A genotype and age are risk factors of T2DM. Meanwhile 25OHD3 may be a preventive factor of T2DM．Conclusions（1）There is a significant correlation between C/A genotype of rs7875232 and the prevalence of T2DM of the Han nationality in Kunming region.（2）25OHD3 is a protective factor of T2DM and the aging is a risk factor of T2DM. |
| Key words: ［Key words］Diabetes mellitus Type 2 Vitamin D receptor gene Polymorphism Polymerase chain reaction of restriction fragment length polymorphism |