| 摘要: |
| ［摘要］ 目的 检测肺结核患者肺泡灌洗液（bronchoalveolar lavage fluid，BALF）及外周血（peripheral blood，PB）中T淋巴细胞亚群，探讨其在肺结核诊断中的价值．方法 选取47例肺结核患者（观察组）及21例健康体检者（对照组）为研究对象，运用流式细胞仪检测其BALF及外周血中T细胞亚群，并比较其差异性．结果 观察组患者BALF及外周血中CD4 比例及CD4/CD8比值均明显低于对照组（P＜0.05）；观察组患者BALF中CD8比例显著高于外周血（P＜0.05）．肺结核患者经治疗2个月后，BALF及外周血中CD4 比例及CD4/CD8比值均有明显升高，BALF中CD8水平与治疗前相比明显降低（P＜0.05）．绘制ROC曲线比较显示，BALF中T淋巴细胞亚群检测价值高于外周血，差异有统计学意义（P＜0.05）．结论 BALF及外周血中T淋巴细胞亚群检测有助于肺结核的诊断及观察疗效． |
| 关键词: ［关键词］ 肺结核 T淋巴细胞亚群 肺泡灌洗液 流式细胞仪 |
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| 基金项目:［基金项目］ 河北省卫生厅医学科学研究重点课题计划基金资助项目（20090264） |
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| The Clinical Value of T Lymphocyte Subsets Analysis with Flow Cytometry in Bronchoalveolar Lavage Fluid of Patients with Pulmonary Tuberculosis |
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LIU Ning1,2
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1.（1）The Chest Hospital of Hebei Province，Shijiazhuang Hebei 050041;2）Dept. of Internal Medicine，The People’s Hospital of Zhengding County，Shijiazhuang Hebei 050800，China）
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| Abstract: |
| ［Abstract］Objective To evaluate the clinical value of T lymphocyte subsets analysis in bronchoalveolar lavage fluid （BALF） and peripheral blood （PB） in patients with pulmonary tuberculosis. Methods Forty-seven patients with pulmonary tuberculosis （observation group） and 21 normal persons （control group） were enrolled in this study. T lymphocyte subsets in BALF and PB of patients were detected by flow cytometry （FCM）， and the differences were compared. Results The CD4 and CD4/CD8 ratios in BALF and PB of observation group were significantly lower than control group （P＜0.05）， and CD8 in observation group was significantly higher in BALF than in PB （P＜0.05）. After 2 months' treatments， the CD4 and CD4/CD8 ratios in BALF and PB of observation group were significantly increase，and the CD8 in BALF was reduced （P＜0.05）. T lymphocyte subsets in BALF showed significantly higher clinical value in diagnosis of pulmonary tuberculosis than that in PB （P＜0.05）， after ROC curve analysis. Conclusion Analysis of T lymphocyte subsets in BALF and PB is helpful for clinic diagnosis and observation of patients with pulmonary tuberculosis. |
| Key words: ［Key words］Pulmonary tuberculosis T lymphocyte subsets Bronchoalveolar lavage fluid Flow cytometry |