| 摘要: |
| ［摘要］目的 探讨苦胆草对博莱霉素致大鼠肺纤维化的防治作用．方法 健康成年SD大鼠40只（200±20）g随机分为4组：正常对照组（未做处理）、生理盐水组（气管滴入生理盐水）、模型组（气管滴入博莱霉素5 mg/kg）和苦胆草干预组［灌胃给予苦胆草片混悬液0.705 g/(100 g.d)，2次/d、共5次，末次给药1 h，气管滴入博莱霉素5 mg/kg］．分别于气管内滴注后14 d和28 d处死动物，检测各项指标．采用碱水解法测定肺组织中羟脯氨酸（HYP）含量，HE染色和银染观察肺组织病理变化．结果 与正常对照组和生理盐水组比较，模型组大鼠肺组织HYP含量明显升高（P＜0.01）；与模型组比较，苦胆草干预组大鼠肺组织HYP含量明显降低（P＜0.01）；病理检查发现，模型组大鼠肺组织有明显肺纤维化样改变，苦胆草干预组可明显减轻肺纤维化程度．结论 苦胆草能降低博莱霉素致肺纤维化大鼠肺组织羟脯氨酸含量，减轻肺纤维化程度，有望成为防治肺纤维化的候选药物． |
| 关键词: ［关键词］苦胆草片 博莱霉素 肺纤维化 羟脯氨酸 龙胆苦苷 |
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| 基金项目:［基金项目］云南省应用基础研究基金资助项目（2014FB152）；云南省中青年学术和技术带头人后备人才项目（2015HB042） |
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| Anti-fibrotic Effect of Kudancao Tablets on Bleomycin-induced Pulmonary Fibrosis in Rats |
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WANG Ying-xia1,2,3
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1.（1）Dept. of Pathology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunan 650032;2）Kunming Medical University，Kunming Yunnan 650500;3）School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Yunnan 650500，China）
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| Abstract: |
| ［Abstract］Objective To investigate the anti-fibrotic effect of Kudancao tablets on bleomycin-induced pulmonary fibrosis in rats . Methods Forty healthy adult SD rats（200±20g）were randomly divided into 4 groups: normal control group（without any treatment），normal saline group（intratracheal instillation of normal saline ），model group（intratracheal instillation of 5mg/kg of Bleomycin）and kudancao tablets group（intragastric administration of kudancao tablets suspended in normal saline， 0.705g/(100 g.d)，2 times/day and for 5 times， then intratracheal instillation of 5 mg/kg of Bleomycin 1h later ）. Rats in all groups were scraficed 14d and 28d after intratracheal instillation. Alkali hydrolysis method was used to detect the HYP content in lung tissues. The pathological changes were observed with HE staining and silver staining. Results Compared with the normal control group and normal saline group， the HYP content in the lung tissues of rats in model group increased significantly（P＜0.01）. Compared with the model group， the HYP content in the lung tissues of rats in kudancao tablets group decreased significantly（P＜0.01）. Pathological examination showed that significant fibrotic changes were found in lungs of rats in model group，while the fibrotic changes were ameliorated in lungs of rats in kudancao tablets group. Conclusion Kudancao tablets may decrease the hydroxyproline content in lung tissues， and ameliorate the fibrotic degree of lungs of rats with bleomycin-induced pulmonary fibrosis. |
| Key words: ［Key words］Kudancao tablets Bleomycin Pulmonary fibrosis Hydroxyproline Gentiopicroside |