| 摘要: |
| ［摘要］目的 探讨金属硫蛋白（metallotionein，MT）基因家族中的MT4基因 G-201A位点单核苷酸多态性（SNPs）在2型糖尿病及原发性高血压的分布差异性．方法 选取2型糖尿病患者324例，原发性高血压患者301例，正常对照者301例．应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测MT4基因 G-201A位点的基因多态性． 结果 （1）2型糖尿病324例、原发性高血压301例、正常对照者301例中MT4 G-201A基因型GG、GA、AA的频率分别为39.5%、46.6%、13.9%；42.2%、48.5%、9.3%；42.5%、51.5%、6.0%；基因型分布均符合Hardy-Weinberg平衡（P＞0.05）；（2）MT4 G-201A多态性：3组间基因型及等位基因（G和A）频率分布差异有统计学意义（P＜0.05）；原发性高血压组和正常组比较分布差异无统计学意义（P＞0.05）；2型糖尿病组和正常组比较分布均差异有统计学意义（P＜0.05）；原发性高血压组与2型糖尿病组比较，基因型及等位基因均差异无统计学意义（P＞0.05）；（3）男性MT4G-201A多态性：3组间基因型及等位基因频率分布差异有统计学意义（P＜0.05）；原发性高血压组与2型糖尿病组比较，基因型及等位基因均无统计学意义；女性MT4 G-201A多态性：3组间基因型及等位基因频率分布，有统计学意义（P＜0.05），2型糖尿病组和正常对照组比较分布均有统计学意义（P＜0.05）；原发性高血压组与2型糖尿病组比较，等位基因频率无统计学意义（P＞0.05）．结论 （1）MT基因G-210A多态性与2型糖尿病相关，与原发性高血压无相关性；（2）女性2型糖尿病MT基因G-210A多态性基因型分布与正常不同，但与高血压无异；男性则无此差异. |
| 关键词: ［关键词］金属硫蛋白基因 多态性 2型糖尿病 原发性高血压 |
| DOI： |
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| 基金项目:［基金项目］国家自然科学基金资助项目（81560072）；云南省科技厅－昆明医科大学联合专项基金资助项目（2014FB037）；云南省卫生厅内设机构项目（2012WS0030） |
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| Difference of Metallothionein Gene G-201A Polymorphism in Type 2 Diabetes and Essential Hypertension |
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NINA Xin1,2,3,4
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1.（1）Dept. of Endocrinology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032;2.２）Dept. of Endocrinology，Central Hospital of Zhengzhou，Zhengzhou Henan 450000;3.３）Dept. of Cardiology;4.４）Dept. of Clinical Laboratory，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China）
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| Abstract: |
| ［Abstract］Objective To investigate the difference of MT4 G-201A of Metallothionein （metallotionein， MT） family gene locus single nucleotide polymorphisms （SNPs） in type 2 diabetes and essential hypertension. Mothods We selected 324 cases of patients with type 2 diabetes， 301 cases of patients with essential hypertension， 301 case of normal physical examination population. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）analysis technology was used to detect MT4 G-201A polymorphism. Results （1） The genotype GG， GA and AA frequency of MT4 G-201A in 324 cases of patients with type 2 diabetes，301 cases of patients with essential hypertension，301 case of normal physical examination population were 39.5%，46.6%，13.9%；42.2%，48.5%，9.3%；and 42.5%，51.5%，6.0%， respectively. The genotypes in all the distribution were in Hardy-Weinberg equilibrium （P＞0.05）.（2） MT4 G-201A polymorphism: Three groups of genotype and allele frequency distribution （G and A） had differences （P＜0.05）; there was no difference between essential hypertension group and normal group （P＞0.05）；there was a difference between type 2 diabetes group and the normal group（P＜0.05）；there was no significant difference in genotype and allele frequency distribution（P＞0.05）.（3）MT4 G-201A polymorphism in Men: Three groups of genotype and allele frequency distribution had differences （P＜0.05）. In essential hypertension and type 2 diabetes group，there was no significant difference in genotype and allele frequency distribution（P＞0.05）.MT4 G-201A polymorphism between women:Three groups of genotype and allele frequency distribution had differences （P＜0.05），there was a difference between type 2 diabetes and normal group genotypes （P＜0.05）. Conclusions （1）There is correlation of MT gene G-210A polymorphism and type 2 diabetes， no association with essential hypertension. （2） There is a difference of MT gene G-210A polymorphism in type 2 diabetes in women， no difference in men. |
| Key words: ［Key words］Metallothionein gene Polymorphism Type 2 diabetes Essential hypertension |