| 摘要: |
| ［摘要］目的 研究云南白药水溶液对牙周膜成纤维细胞C-fos、C-jun基因表达的影响，探讨云南白药水溶液促进人牙周膜成纤维细胞（HPDLFs）增殖和成骨分化的可能机制．方法 原代培养HPDLFs，取第4～6代细胞用于实验．分为空白对照组，云南不同浓度云南白药水溶液组，阳性对照组．各组细胞与云南白药水溶液共培养4h，采用反转录聚合酶链反应检测C-fos和C-jun mRNA的表达．结果 RT-PCR 的结果说明，与空白对照组相比，云南白药水溶液能促进HPDLFs细胞C-fos和C-jun mRNA 的表达．且促进作用呈浓度依赖性，与对照组比较，各组差异有统计学意义（P＜0.05）．结论 云南白药水溶液能够上调HPDLFs中C-fos、C-jun的表达．云南白药水溶液可能通过上调C-fos、C-jun的表达促进HPDLFs的增殖和成骨分化，促进骨形成． |
| 关键词: ［关键词］云南白药 人牙周膜成纤维细胞 C-fos C-jun 增殖分化 |
| DOI： |
| 分类号: |
| 基金项目:［基金项目］云南省卫生科技计划基金资助项目（2012WS0112） |
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| Effects of Yunnan BaiYao on the Expressions of c-fos and C-jan mRNA in Periodontal Ligament Cells in Vitro |
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CHU Wen
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（Dept. of Oral Preventive Medicine，The 2nd People’s Hospital of Yunnan Province，Kunming Yunnna 650021，China）
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| Abstract: |
| ［Abstract］Objective To study the effects of Yunnan BaiYao on the expressions of c-fos and c-jun mRNA in HPDLFs and explore the potential mechanism that Yunnan BaiYao promotes the proliferation and differentiation of HPDLFs．Methods The HPDLFs tissue was obtained from the extracted healthy premolar．The HPDLFs used underwent four to six passages．Cells were divided into untreated group， positive control group and treated group. In treated group，HPDLFs were co-cultured with Yunnan BaiYao solution for 4h with gradient concentration. The expressions of c-fos and c-jun mRNA were determined by RT-PCR．Results The results showed that the expressions of c-fos and c-jun mRNA were up-regulated significantly in treated group compared with control group（P＜0.05）．Conclusion Yunnna BaiYao can upregulate the expressions of c-fos and C-jun mRNA in HPDLFs. Through upregulating the expressions of c-fos and c-jun mRNA in hPDLs，Yunnan BaiYao can promote proliferation and differentiation of ossification of HPDLFs to induce bone formation. |
| Key words: ［Key words］Yunnna BaiYao Human periodontal ligament cells C-fos C-jun Proliferation and differentiation |