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| 人脐带间充质干细胞的培养鉴定及其向神经元细胞分化的研究 |
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张济周,李青,林萍,杨梁梓,宋精玲
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1. 温州市中医院肿瘤科,浙江,温州,325000
2. 昆明医学院第二附属医院中西医结合科,云南,昆明,650101
3. 昆明医学院病理教研室,云南,昆明,650031
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| 摘要: |
| ［摘要］目的 研究脐带间充质干细胞（UC-MSCs）在体外分离、培养、扩增和鉴定以及向神经元分化的方法．方法 30例符合入选标准的脐带采用贴壁法分离得到UC-MSCs，选用20% FBS血清培养液进行原代培养，再选用10% FBS扩增培养液进行一般传代．用神经分化培养基诱导UC-MSCs向神经细胞分化，在荧光显微镜下观察细胞形态， 透射电镜观察UC-MSCs超微结构，用流式细胞仪分析其表面特性，采用直接和间接免疫荧光法检测细胞悬浮液的表型特征，用抗人神经纤维 M、突触素、微管蛋白、半乳糖神经酰胺的单克隆抗体检测各代之间神经诱导形成率．结果 12 h后细胞开始贴壁生长，超微结构观察表现出未分化细胞的特征，CD123、CD49、CD29、CD73和CD166均为阳性，免疫荧光检测人UC-MSCs分化成神经细胞．结论 UC-MSCs长期传代培养，生物学特性稳定，具有向神经细胞分化的潜能 |
| 关键词: ［关键词］脐带间充质干细胞 神经细胞 分化 |
| DOI： |
| 分类号:R285.5 |
| 基金项目:云南省卫生厅科研基金资助项目(2009NS065) |
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| Pathological Study of PNS in Regulating Cathepsin B Expression in Rabbits with Pulmonary Fibrosis of Pulmonary Heart Disease |
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ZHANG Ji-zhou,LI Qing,LIN Ping,YANG Liang-zi,SONG Jing-ling
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ZHANG Ji-zhou1),LI Qing2),LIN Ping3),YANG Liang-zi2),SONG Jing-ling3)(1)Dept.of Tumour,Wenzhou Chinese Medicine Hospital,Wenzhou Zhejiang 325000,2)Dept.of Integrative Medicine,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,3)Dept.of Pathology,Kunming Medical University,Kunming Yunnan 650031,China)
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| Abstract: |
| Objective To investigate the effect of PNS in regulating the expression of Cathepsin B in rabbits with pulmonary fibrosis of pulmonary heart disease from the perspective of pathology.Methods Forty healthy Japanese rabbits were randomly divided into five groups(eight rabbits in each group):the control group,the PF model group,the PNS prevention group,treatment group and the western medicine treatment group.The expression of CB protein and the pathological structural changes in lung tissue were observed under... |
| Key words: PNS Pulmonary heart disease Pulmonary fibrosis Rabbit Cathepsin B Expression regulation Pathology |