| 摘要: |
| ［摘要］目的 研究G蛋白α亚基Gnaq对人神经母细胞瘤细胞株SH-SY5Y增殖的影响及其可能机制．方法 利用慢病毒转染技术在SH-SY5Y细胞中过表达Gnaq，利用荧光显微镜检测转染效率，利用逆转录PCR检测过表达后Gnaq的转录水平，利用MTT技术检测过表达Gnaq后细胞增殖速率的变化，同时利用免疫印迹实验检测转录因子NF-κB的表达水平的变化，分析Gnaq影响SH-SY5Y细胞增殖速率的可能机制．结果 （1）慢病毒载体对SH-SY5Y细胞的转染效率达（97.60±2.14）%；（2）过表达Gnaq基因后，SH-SY5Y细胞中的Gnaq转录水平明显升高，细胞的增殖速率明显加快，NF-κB表达水平明显上调（P＜0.05）．结论 Gnaq基因通过调节NF-κB信号通路而对SH-SY5Y细胞发挥明显的促增殖作用． |
| 关键词: ［关键词］Gnaq NF-κB SH-SY5Y细胞 增殖 机制 |
| DOI： |
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| 基金项目:［基金项目］国家自然科学基金资助项目（81260195） |
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| Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action |
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JIA Nan－nan1,2,3
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1.（1）Dept. of Anatomy and Histoembryology，Kunming Medical University，Kunming Yunnan 650500;2） Dept. of the Head and Neck Surgery，The Third Affiliate Hospital of Kunming Medical University，Kunming Yunnan 650118;3）School of International Education，Kunming Medical University，Kunming Yunnan 650500，China）
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| Abstract: |
| ［Abstract］Objective To study the role of Gnaq，the α subunit of G proteins，in the proliferation of neuroblastoma cell line SH-SY5Y，and further explore the underlying mechanism. Methods Over-expression of Gnaq in SH-SY5Y cells was performed by lentivirus infection. The infection rate was checked by fluorescence microscope. Then MTT experiment was used to evaluate the proliferation of the Gnaq-overexpressed cells. The transcription level of Gnaq and expression level of nuclear factor kappa B （NF-κB）were measured by PCR and Western Blot respectively. Results （1）The infection rate of lentivirus in SH-SY5Y cells was（97.60±2.14）%. （2）The proliferation and NF-κB level of SH-SY5Y cells was significantly upregulated with Gnaq over-expression. Conclusions Gnaq plays an important role in the proliferation of SH-SY5Y cells by regulating the NF-κB signal pathway. |
| Key words: ［Key words］Gnaq NF-κB SH-SY5Y cell Proliferation Mechanism |