| 摘要: |
| ［摘要］目的 对扶正固本膏对大鼠下丘脑-肾上腺皮质系统的影响进行研究，以探讨扶正固本膏是否对丘脑-肾上腺皮质系统具有影响作用．方法 用大鼠灌胃给予氢化可的松造成肾阳虚模型同时，每周称量体重一次，连续造模给药35 d；造模17 d后，在大鼠左右前肢蹊部各植入1个20 mg灭菌棉球；给药结束后，后麻醉大鼠，取下左侧肾上腺，立即用ACTH攻击，15 min后取下右侧肾上腺；检测血浆CORT；取出棉球肉芽、胸腺、肾上腺称量，并检测左右肾上腺Vc含量，计算其差值，肉芽70℃干燥12 h，对结果进行统计分析．结果（1）从造模第1周以后，模型组与给药组动物增长明显下降，动物出现明显被毛枯萎无光泽、弓腰等肾阳虚症状；（2）给药35 d后，给药35 d后，扶正固本膏各剂量组对大鼠胸腺有明显抑制作用；与空白组比较棉球肉芽干重、湿重明显降低，与模型比较，无明显差异，但显示出扶正固本膏对氢化可的松一定的协同作用；与空白组比较，模型组、阳性对照、扶正固本膏肾上腺明显降低，与模型比较阳性对照、扶正固本膏对肾上腺降低有明显抑制作用；（3）阳性对照组和扶正固本膏各剂量组对大鼠左右肾上腺Vc含量差值显著提高，而对CORT有明显增高作用．结论 扶正固本膏对大鼠的免疫系统、下丘脑-肾上腺皮质系有统明显影响作用． |
| 关键词: ［关键词］肾阳虚 肾上腺 大鼠 |
| DOI： |
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| 基金项目:［基金项目］云南省科技厅社会发展科技计划项目（2012CG031） |
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| Effect of Fuzhengguben Jelly on Hypothalamus-adrenal Cortex System of Rats |
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ZHANG Hong-xiang1,2
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1.（1）Yunnan Kunming Blood Center，Kunming Yunnan 650116;2.２）Yunnan Institute of Materia Medica，Kunming Yunnan 650111，China）
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| Abstract: |
| ［Abstract］Objective To investigate the effect of Fuzhengguben Jelly on rat hypothalamus-adrenal cortex system．Methods SD rats were administered with hydrocortisone to induce kidney yang deficiency and Fuzhengguben jelly for treatment for continuousl 35 days. The body weight was weighed every one week. On day 17， 10-mg sterile cotton balls were implanted in both forelimb footpaths of the animals. At the end of the experiment， the rats were anesthetized and the the left adrenal glands removed followed by ACTH attack immediately. After another 15 min，the right adrenal glands were removed. Then the blood was collected and plasma CORT was measured. The tampon granulation，thymus，adrenal gland were also removed and weighed. Meanwhile， the difference in Vc content of the both adrenal glands was analyzed. Results（1）From the first week after modeling， the weights of the animals in model group and treatment group were significantly decreased， and dull hair and hunch back were observed，suggesting a obvious symptoms of kidney yang deficiency.（2）After treatment for 35 days， the various doses of Fuzhengguben jelly markedly suppressed rat thymus. There were no significant differences in tampon granuloma dry and wet weight between the model group and the Fuzhengguben jelly group. （3）In addition， Fuzhengguben jelly and positive drug remarkably elevated the Vc content difference between the left and the right adrenal gland，and had significant effect on CORT. Conclusions Fuzhengguben jelly has significant effects on immune and hypothalamus-adrenal cortex systems of the rats. |
| Key words: ［Key words］Kidney yang deficiency Adrenal gland Rats |