| 摘要: |
| ［摘要］目的 探索调控内源性大麻素系统对酒精诱导肝细胞损伤模型的影响．方法 采用CCK-8法筛选适宜酒精浓度，建立酒精诱导的L-02人肝脏细胞和HepG2人肝癌细胞损伤模型；筛选对细胞活性无影响的URB937浓度范围；应用酒精性肝细胞损伤模型，评估URB937对酒精损伤下肝细胞的影响．结果 0.1～50 μmol/L浓度范围内，URB937对L-02和HepG2细胞的增殖活性无抑制作用（P＞0.05）；相同浓度范围的URB937合并酒精（100 mmol/L或500 mmol/L）处理，增殖活性抑制显著，酒精诱导的细胞损伤加重，部分细胞出现坏死（P＜0.05）． 结论 升高内源性大麻素水平将加重酒精诱导的细胞损伤． |
| 关键词: ［关键词］酒精损伤 人肝脏细胞 人肝癌细胞 内源性大麻素系统 |
| DOI： |
| 分类号: |
| 基金项目:［基金项目］国家自然科学基金资助项目（81160035） |
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| The Effects of Endocannabinoid System on Alcohol-induced Liver Cell Injury |
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ZHAO Xiao-lin1,2,3,4
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1.（1）West China School of Pharmacy，Sichuan University，Chengdu Sichuan 610041;2.２）Laboratory of Anesthesia and Critical Care Medicine，Translational Neuroscience Center，West China Hospital of Sichuan University，Chengdu Sichuan 610017;3.３）Dept. of Anesthesiology，The First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101;4.４）Dept. of Anesthesiology，West China Hospital of Sichuan University，Chengdu Sichuan 610041，China）
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| Abstract: |
| ［Abstract］Objective To explore the effects of endocannabinoid system on alcohol-induced liver cell injury. Methods Alcohol-induced liver cell injury model was established by incubating human liver cell（L-02）and human hepatoma cell（HepG2）with the medium containing alcohol. We screened URB937 concentration range， and evaluated its effecst on alcohol-induced liver cell injury. Results Treatment with URB937（0.1～50 μmol/L） alone didn’t affect L-02 and HepG2 cell viability（P＞0.05）. Under the situation of alcohol-induced liver cell injury， URB937 co-treatment induced significant cell death and necrosis（P＜0.05）. Conclusions Elevation of endogenous anandamide concentration would aggravate the cell injury induced by alcohol. |
| Key words: ［Key words］Alcohol-induced injury Human liver cell Human hepatoma cell Endocannabinoid system |