| 摘要: |
| ［摘要］目的 比较癌组织与正常组织中SOX4、BRMS-1、CYTB、P53表达的差异，了解乳腺癌与表达的关系．方法 通过SYBR Green实时荧光定量PCR方法检测乳腺癌组织和正常乳腺组织中SOX4、BRMS-1的含量． 结果 （1）SOX4和CYTB在乳腺癌组织中表达升高（P＜0.05），而BRMS-1在乳腺癌组织中表达降低（P＜0.05）；（2）在癌组织中SOX4和BRMS-1的表达存在正相关关系（r = 0.57，P＜0.05）；在正常组织中SOX4和CYTB的表达存在负相关关系（r = -0.64，P＜0.05）．结论 SOX4在乳腺癌组织中处于高表达状态，并与BRMS-1和CYTB基因表达有关． |
| 关键词: ［关键词］乳腺癌 SOX4 BRMS-1 CYTB |
| DOI： |
| 分类号: |
| 基金项目:［基金项目］国家自然科学基金资助项目（81460491） |
|
| The mRNA Expression of SOX4， BRMS-1 in Breast Cancer and Their Relationship |
|
HE Yue-feng1,2
|
|
1.（1）Kunming Medical University，Kunming Yunnan 650500;2）Dept. of General Surgery，Kunming General Hospital of Chengdu Military Region，Kunming Yunnan 650031，China）
|
| Abstract: |
| ［Abstract］Objective To study the expression characteristics of SOX4， BRMS-1， CYTB and P53 in breast cancer. Method We selected 35 patients with breast cancer and collected their cancer tissue and adjacent normal tissue，used TRIzol to extract total RNA and real-time quantitative PCR to detect relative gene expression levels. Results The relative expression levels of SOX4 and BRMS-1 in cancer tissue were significantly higher than in adjacent normal tissue（P＜0.05）. The relative expression levels of BRMS-1 in cancer tissue were significantly lower than in adjacent normal tissue（P＜0.05）.There was a significant correlation between the expression of SOX4 and BRMS-1 （r＝0.57，P＜0.05）in cancer tissue and a significant negative correlation between the expression of SOX4 and CYTB in adjacent normal tissue（r＝-0.64，P＜0.05）. Conclusion The relative expression levels of SOX4 in cancer tissue are significantly high and have a significant correlation with BRMS-1，CYTB in breast cancer. |
| Key words: ［Key words］Breast cancer SOX4 BRMS-1 CYTB |