| 摘要: |
| ［摘要］目的 检测不同年龄组雄性SD大鼠血调节性T细胞（Treg）数量占CD4+T细胞百分比，了解Treg细胞对老年性骨质疏松症的影响．方法 选取6～8周龄、6月龄、12月龄、24月龄雄性SD大鼠每组各10只作为研究对象，采用双能X线吸收仪（DEXA）检测大鼠股骨、脊柱、骨盆、全身的骨密度；大鼠心脏采血，采用CD25+联合FoxP3+表达来描述Treg细胞，流式细胞仪测定血Treg数量占CD4+T细胞的百分比．结果（1）6～8周龄组、6月龄组、12月龄组、24月龄组Treg数量不同，差异有统计学意义（P＜0.01）．6～8周龄组Treg数量百分比低于6月龄组、12月龄组、24月龄组，差异有统计学意义（P＜0.01）；6月龄组Treg数量百分比低于12月龄组、24月龄组（P＜0.05）；24月龄组Treg数量百分比低于12月龄组比较，但差异无统计学意义（P＞0.05）．（2）Treg数量占CD4+T细胞百分比与股骨、脊柱、骨盆、全身骨密度值均成正相关，差异均有统计学意义（P＜0.01）．结论（1）6～8周龄组、6月龄组、12月龄组、24月龄组Treg数量占CD4+T细胞百分比不同，6～8周龄时最低，6月龄时开始升高，12月龄时达到最高值，24月龄时出现下降，提示不同年龄组大鼠Treg数量占CD4+T细胞有明显差异（P＜0.05）.（2）Treg数量占CD4+T细胞百分比与检测部位骨密度值呈正相关性，提示随着Treg数量百分比的增高，骨吸收减少，骨密度值升高，随着Treg数量百分比的降低，骨密度降低，鉴于随着年龄的增长，Treg数量出现下降，推测外源性注射Treg可抑制破骨细胞性骨吸收，可成为治疗老年性骨质疏松的新方向. |
| 关键词: ［关键词］骨质疏松症 调节性T细胞 老年 |
| DOI： |
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| 基金项目:［基金项目］云南省内设研究机构科技计划项目（2012ws0031） |
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| The Relationship of Regulatory T Cells and Senile Osteoporosis |
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JIANG Yan1,2,3
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1.（1）Dept. of Endocrinology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032;2）Dept.of Endocrinology，People’s Hospital of YUXI City，Yunnan 653100;3）Dept. of Laboratory Medicine，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China）
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| Abstract: |
| ［Abstract］Objective To understand the effect of regulatory T cells on senile osteoporosis by measuring the percentage of regulatory T cells in CD4+T cells in different age groups of male SD rats. Methods Sprague-Dawley male rats which was 6-8 weeks，6 months，12 months，24 months old were selected as subjects. There were 10 rats in each group. We detected bone mineral density of femur，spine，pelvis，whole-body of rats by dual-energy X -ray absorptiometry. We collected blood samples from the heart，used CD25/forkhead box P3 double-positive to describe regulatory T cells and then measured the percentage of regulatory T cells in CD4+T cells by flow cytometry. Results（1）The percentage of regulatory T cells in CD4+T cells in 6-8 weeks，6 months，12 months，24 months old male rats groups were not the same， the difference was statistically significant（P＝0.000）. The percentage of regulatory T cells in CD4+T cells of 6-8weeks old groups was lower than 6 months，12 months，24months old groups， the difference was statistically significant（P＝0.000）. The percentage of regulatory T cells in CD4+T cells of 6 months old groups was lower than 12 months，24 months old groups， the difference was statistically significant（P＜0.05）. The percentage of regulatory T cells in CD4+T cells of 24 months old groups was lower than 12months， but there was no significant difference（P＞0.05）.（2）There was a positively correlation between the percentage of regulatory T cells in CD4+T cells and bone mineral density of femur，spine，pelvis，whole-body. Conclusions （1）The percentage of regulatory T cells in CD4+T cells in four groups are not the same，the lowest at 6-8 weeks of age，by 6 months，there is an increase and when 12 months increase to a maximum then decrease at 24 months of age，indicating that the percentage of regulatory T cells in CD4+T cells in different age groups are significantly different. （2）There is a positive correlation between the percentage of regulatory T cells in CD4+T cells and bone mineral density of femur，spine，pelvis，whole-body，indicating that because of the bone resorption reducing with the percentage of regulatory T cells increasing，the values of bone mineral density raising，when the percentage of regulatory T cells decreasing，the values of bone mineral density declining. In view of the number of regulatory T cells declining with aging，suggesting that exogenous injection of regulatory T cells may inhibit the bone resorption by osteoclast，which may become the new direction of senile osteoporosis treatment. |
| Key words: ［Key words］Osteoporosis Regulatory T cells Aging |