| 摘要: |
| ［摘要］目的 对地衣抗癌活性部位AMH-T进行常规毒理学试验研究，了解其毒作用的性质和强度，为抗癌药物的开发提供安全性依据．方法 急性毒性试验、小鼠骨髓微核试验、小鼠精子畸形试验、Ames试验．结果 雄性小鼠经皮下注射给药的LD50为147 mg/kg，雌性小鼠的LD50为171 mg/kg．小鼠骨髓微核试验、小鼠精子畸形试验和Ames试验在试验剂量下未见阳性结果．结论 AMH-T皮下注射给药具有较强的急性毒性和肝毒性，实验剂量范围内未见遗传毒性． |
| 关键词: ［关键词］地衣 抗癌药物 急性毒性 遗传毒性 毒理学 |
| DOI： |
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| 基金项目:［基金项目］国家自然科学基金资助项目（30960437）；云南省科技计划基金资助项目（2009FXL001）；云南省科技厅-昆明医科大学应用基础研究联合专项基金重点资助项目（2012FB001） |
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| Toxicological Study on the Anticancer Bioactive Fraction AMH-T of Lichen |
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HE Yun-fa1,2
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1.（1）School of Public Health，Kunming Medical University，Kunming Yunnan 650500;2）Yunnan Center for Disease Prevention and Control，Kunming Yunnan 650000，China）
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| Abstract: |
| ［Abstract］Objective To provide safety reference for the development of anti-cancer drugs by evaluating thetoxicological safety of the anticancer bioactive fraction AMH-T of lichen through the understanding of its poisonous nature and the intensity. Methods Acute toxicity test， bone marrow micronucleus test in mice，sperm malformation test in rats， Ames test and short-term repeat drug test in mice were conducted. Results Male mice were injected LD50 of 147 mg/kg and female mice 171 mg/kg. Conclusion Injection of AMH-T has acute toxicity and liver toxicity，but has no genetic toxicity. |
| Key words: ［Key words］Lichen Anti-cancer drug Acute toxicity Genotoxicity Toxicology |