| 摘要: |
| ［摘要］目的 使用Quantitative Real-time PCR（q-PCR）检测大鼠脑缺血再灌注后不同时间点TNF-α，IL-6和IL-1β基因的表达变化．方法 成年雄性SD大鼠24只随机分为4组：假手术组，脑缺血再灌注损伤3 h组，脑缺血再灌注损伤6 h组和脑缺血再灌注损伤12 h组．各组大鼠于再灌注后相应各时间点取缺血侧大脑皮质进行q-PCR检测，对TNF-α，IL-6和IL-1β基因表达进行定量分析．结果 假手术组TNF-α，IL-6和IL-1β基因mRNA水平较低，脑缺血再灌注损伤后明显升高后又逐渐降低．TNF-α基因mRNA表达于再灌注损伤后3 h显著升高并达峰值（P＜0.01），损伤后12 h又呈显著下降（P＜0.01）；IL-6基因mRNA表达于再灌注损伤后3 h明显升高，6 h达峰值（P＜0.01），12 h时较6 h又呈显著下降（P＜0.01）；IL-1β基因mRNA表达于再灌注损伤后3 h升高，6 h达峰值（P＜0.01），损伤后12 h呈现显著下降（P＜0.01）．结论 大鼠脑缺血再灌注损伤后，TNF-α，IL-6和IL-1β mRNA表达水平在再灌注损伤早期显著升高，达高峰后又逐渐下降，TNF-α，IL-6和IL-1β基因在脑缺血再灌注损伤后的表达变化为脑缺血再灌注损伤的机制研究提供了理论依据． |
| 关键词: ［关键词］脑缺血 再灌注损伤 实时荧光定量聚合酶链反应 炎症因子 |
| DOI： |
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| 基金项目:［基金项目］云南省科技厅应用基础研究基金资助项目（2016NS043） |
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| The Gene Expression Change of Inflammatory Factors TNF-α， IL-6 and IL-1βin Cerebral Ischemia-Reperfusion Rats |
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LI Jun-jie
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（Dept. of Anesthesiology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China）
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| Abstract: |
| ［Abstract］Objective To investigate the gene expression change of TNF-α， IL-6 and IL-1β at different time points in brain tissues of rats with cerebral ischemia reperfusion injury. Methods A total of 24 adult male SD rats were randomly divided into four groups: sham group and 3 groups with brain ischemia reperfusion of 3h， 6h and 12h. Real-Time PCR was used to analyze the gene expression of TNF-α， IL-6 and IL-1β at 3h， 6h， and 12h after reperfusion. Results In the sham group， the mRNA expression levels of TNF-α， IL-6 and IL-1βin were low， but increased immediately after brain ischemia injury and decreased gradually thereafter. The gene expression of TNF-α mRNA at 3h after reperfusion was significantly increased and reached the peak （P＜0.01） then significantly decreased at 12h after reperfusion. The gene expression of IL-6 mRNA was notably increased at 3h after reperfusion and peaked at 6h （P＜0.01）， and significantly decreased at 12h compared with 6h （P＜0.01）. The gene expression of IL-1βmRNA at 3h after reperfusion was significantly increased， peaked at 6h （P＜0.01） and significantly decreased at 12h （P＜0.01）. Conclusion The gene expression levels of TNF-α， IL-6 and IL-1β mRNA increased significantly in the early stage of reperfusion and decreased gradually after reaching the peak， which suggested that the gene expression change of TNF-α，IL-6 and IL-1β was involved in the mechanism of cerebral ischemia reperfusion injury. |
| Key words: ［Key words］Cerebral ischemia Reperfusion injury Quantitative Real-Time PCR Inflammatory factors |